C5870-01G-HRP

Clusterin (Clusterin Precursor, CLU, AAG4, Apolipoprotein J, APOJ, Apo-J, CLI, Complement-associated Protein SP-40, Complement Cytolysis Inhibitor, KUB1, MGC24903, NA1/NA2, SGP2, SGP-2, SP-40, Testosterone-repressed Prostate Message 2, TRPM2, TRPM-2) (HRP)

Size

£1,119.00

SKU:: C5870-01G-HRP
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Glomerular basement membrane from a post-mortem kidney.
Clone:: 3R3/2
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Extra Details:: Clusterin is an ubiquitously expressed protein of two 40-kD chains, alpha and beta, covalently joined by disulfide bonds. It exists in two forms; a pro-apoptotic form localized to the nucleus and a pro-survival secreted form. Both are thought to be involved in DNA repair and cell cycle regulation. The secreted form is thought to inhibit apoptotic signalling by BAX, Clusterin suppression can sensitize cells to chemotherapy. Clusterin is overexpressed in human prostate cancer, breast cancers and squamous cell carcinomas. Part of the SC5b-9 complex, clusterin prevents the binding of a C5b-C7 complex to the membrane of the target cell inhibiting the complement cascade. Clusterin acts as an extracellular chaperone inhibiting the formation of human lysozyme amyloid associated with Alzheimer’s disease. Clusterin is also important in spermatogenesis and is secreted by Sertoli cells. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher. Positive Control: Kidney from post streptocial glomerulonephritis patients. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice