C5851-95L

Clostridium difficile Toxin A (tcdA, toxA)

Size

£544.00

SKU:: C5851-95L
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Highly purified Toxin A from Clostridium difficile.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.075% sodium azide.
Extra Details:: Clostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cuase diarrhea and inflammation of the large intestine. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Direct ELISA: 1:20000 using 2ug/ml coating of Toxin A Western Blot: 1:4000 for 1ug of Toxin A Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906-10: IgY, Chicken (HRP) Rb x Ch I1906-12: IgY, Chicken (FITC) Rb x Ch I1906-14: IgY, Chicken (AP) Rb x Ch Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.