C5851-95-ML650
Clostridium difficile Toxin A (tcdA, toxA) (MaxLight 650)
Size
£832.00
- SKU:
- C5851-95-ML650
- Application:
- FLISA
- Purity:
- Purified by Protein A affinity chromatography from culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Clostridium difficile toxin A.
- Clone:
- 5E245
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Clostridium difficile is a gram-positive motile bacterium which is the leading cause of diarrhea in developed countries. The incidence of disease in humans varies greatly with age, spore density and the administration of antibiotics. Toxin A (TcdA) is a large exotoxin which is an essential virulence factor for C. difficile infection. TcdA has a molecular weight of 308kD and is a glucosyltransferase, which irreversibly inactivates the Ras family of small GTPases to disrupt many important signalling pathways. Applications: Suitable for use in FLISA. Other applications not tested. Recommended Dilution: FLISA: 1:20-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice