C5851-95-BIOTIN
Clostridium difficile Toxin A (tcdA, toxA) (Biotin)
Size
£832.00
- SKU:
- C5851-95-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography from culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Clostridium difficile toxin A.
- Clone:
- 5E245
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- Clostridium difficile is a gram-positive motile bacterium which is the leading cause of diarrhea in developed countries. The incidence of disease in humans varies greatly with age, spore density and the administration of antibiotics. Toxin A (TcdA) is a large exotoxin which is an essential virulence factor for C. difficile infection. TcdA has a molecular weight of 308kD and is a glucosyltransferase, which irreversibly inactivates the Ras family of small GTPases to disrupt many important signalling pathways. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:20-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice