C5851-82H

Clostridium botulinum E Toxoid

Size

£557.00

SKU:: C5851-82H
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide analog of the amino terminal region of BoTox-E.
Clone:: 7C223
Format:: Supplied as a liquid.
Extra Details:: Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, ELISA and Inhibition of BoTox-E entry into Neuro2A Cells. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:25. Inhinition: Inhibits the entry of BoTox-E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin. Optimal dilutions to be determined by the researcher. Immunofluorescent Staining of Neuro2A Cells: 1. Neuro2A cells treated with GT1b ganglioside. 2. Following the treatment, the cells were washed x 4, fixed in neutral buffered formalin for 10 min, permeablized and blocked. 3. Incubate for 60 minutes with the C5851-82H diluted 1:25. 4. Incubate with IgM (FITC) GoatxMouse. The immunofluorescent staining pattern was observed using epifluorescent microscopy. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.