C5851-48B
Clostridium botulinum B Toxoid
Size
£557.00
- SKU:
- C5851-48B
- Purity:
- Supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide analog of the carboxyl terminal region of BoTox-B.
- Clone:
- 7C221
- Format:
- Supplied as a liquid.
- Extra Details:
- Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, RIA, ELISA and Inhibition of BoTox-B binding to Neuro2A Cells. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:10 stains Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin (BoTox-B) using an indirect staining procedure. Following treatment, the cells were washed 4 times, fixed in a neutral buffered formalin for 10 minutes, permeablized and blocked. Reacted with C5851-48B for 60 minutes and then probed with a secondary antibody; IgG (FITC) Pab Gt x Mo. Epifluorescent microscopy was used to observe staining pattern. Inhibition of BoTox-B binding to Neuro2A Cells: Inhibits the binding of BoTox-B to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin, and prior incubation of C5851-48B with the synthetic peptide immunogen has been shown to eliminate the inhibition of cellular binding. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice