C5841

CLIP (Class II-associated Invariant Chain Peptide, CD74, HLA class II histocompatibility Antigen gamma Chain, HLA DR Antigens Associated invariant Chain, HLA DR gamma, HLA-DR Antigens-Associated invariant Chain, HLA-DR-gamma, HLADG, HLADR Antigens Associated invariant Chain)

Size

£402.00

SKU:: C5841
Purity:: Purified from ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to a portion of human CLIP.
Clone:: 4E49 (CerCLIP.1)
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: CLIP (class II-associated invariant chain peptide) is an important peptide in the MHC-II antigen processing pathway, formed by the enzymatic cleavage of amino acids 81-104 from the invariant chain. During MHC-II synthesis in the ER and transport to endocytic vesicles, the antigen binding site of MHC-II is obstructed by the presence of the invariant chain. As the MHC-II complex moves through the Golgi, the invariant chain is degraded, leaving the CLIP fragment in the MHC-II peptide binding groove. CLIP is eventually displaced by the loading of antigenic peptides, followed by presentation on the APC cell surface to CD4+ T-cells. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.