C5839-74A
CLEC16A (C-type Lectin Domain Family 16, Member A, Gop-1, KIAA0350, KIAA0350 Protein, MGC111457, Uncharacterized Protein KIAA0350)
Size
£642.00
- SKU:
- C5839-74A
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Synthetic peptide coresponding to EEAACAEPVGTAED, from the C-Terminus of human CLEC16A (NP_056041.1)
- Format:
- Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
- Extra Details:
- Applications: Suitable for use in Peptide ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:1000 Western Blot:1-3ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human Kidney and Liver lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See C5839-74A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
- Shipping Conditions:
- Blue Ice