C5839-66
CLEC4E (C-type lectin domain family 4, member E)
Size
£442.00
- SKU:
- C5839-66
- Purity:
- Purified by Protein G affinity chromatography. (≥95% SDS-PAGE)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to aa41-219 from human CLEC4E expressed in E. coli.
- Clone:
- 10K398
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
- Extra Details:
- CLEC4E is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. CLEC4E expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6,6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB). Recently it was reported that CLEC4E is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor gamma-chain (FcR-gamma) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:50. Incubated for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB). Immunofluorescence (IF-IC): 1:500 Optimal dilutions to be determined by the researcher. Hybridoma: Mouse F0 myeloma cells with spleen cells from BALB/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice