C5838-61A
Claudin 12 (CLDN12)
Size
£832.00
- SKU:
- C5838-61A
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Thr244 from human Claudin 12, expressed in HEK293 human embryonic kidney cell line (NP_036261). Species Sequence Homology: mouse and rat: 92%
- Clone:
- 7H195
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Claudin-12 is a 244 amino acid multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin-12 is expressed on intestinal, bladder, and blood-brain barrier epithelial cells. Human Claudin-12 shares 92% aa sequence identity with mouse and rat Claudin-12. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/ml labels 1x10e6 cells. Validated for use with CaCo2 human colorectal adenocarcinoma cell line. Immunocytochemistry: 8-25ug/ml in immersion fixed NS0 mouse myeloma cell line transfected with human Claudin 12 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice