C5838-61
Claudin 12 (CLDN12)
Size
£761.00
- SKU:
- C5838-61
- Purity:
- Purified by immunoaffinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide derived from the N-terminal region of mouse and human Claudin 12
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
- Extra Details:
- Tight junctions are intercellular junctions that act as both the barrier (or gate), and the fence. The barrier function of tight junctions regulates the passage of ions, water, and various macromolecules through paracellular spaces. The fence function maintains cell polarity, thus, tight junctions work as a fence to prevent intermixing of molecules in the apical membrane with those in the lateral membrane. Tight junctions are comprised of the membrane proteins occludin, claudins, and JAMs. Claudins are responsible for the formation of tight junction strands and are connected with the actin cytoskeleton mediated by ZO-1. Claudin 12 expression has been observed in the lung, brain, kidney, skin, urinary bladder and ear. Applications: Suitable for use in Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-3ug/ml Immunofluorescence (IC): 1:100-1;500 Optimal dilutions to be determined by the researcher. Positive Control: Mouse lung, colon, and kidney homogenates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice