C5838-35A-ML650
Claudin 4 (CLDN4) (MaxLight 650)
Size
£1,081.00
- SKU:
- C5838-35A-ML650
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin 4.
- Clone:
- 6A43
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Initially only transmembrane protein known to be associated with tight junctions was occludin, an ~65kD protein with four transmembrane domains. Despite widespread expectation, a critical structural role for occludin in TJ strands was ruled out by the observation of apparently normal tight junctions formed between cells disrupted at both occludin alleles. A closer examination of isolated tight junctions uncovered two related ~22kD, four-transmembrane domain proteins, claudin 1 and claudin 2, with no similarity to occludin. Claudin 1 and 2 induce networks of strands characteristic of true tight junctions. Though inconclusive, these findings suggest that claudin 1 and 2 are major structural components of TJ strands and that occludin plays some other accessory role. Excitement in the tight junction field continues to rise following the recent discovery of claudins 3, 4, 5, 6, 7 and 8 and experiments suggesting that tight junctions in different tissues are comprised of different sets of claudin family proteins. The overexpression of Claudin-4 was found to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant change for Cl-. Claudin-4 is the first to confer ionic selectivity to paracellular transport, leading to the prediction that the combination of different claudins defines the overall selectivity of different junctions. Thus, Claudin-4 forms channels through the tight junctions that discriminate against Na+ ions and are indifferent to Cl- ions. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (FFPE): Heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice