C5838-16
Claudin 2 (CLDN2)
Size
£800.00
- SKU:
- C5838-16
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to a 26 amino acid sequence at the C-terminus of mouse Claudin-2 protein.
- Clone:
- 3G95
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- Tight Junctions (TJ) are specialized membrane domains that seal the intercellular spaces in epithelia and endothelia and thus contribute to the permeability barrier between lumenal and interstitial compartments. Tight junctions not only separate distinct physiological compartments, but they also confer selectivity to the transepithelial flux of molecules and ions through the intercellular spaces between the epithelial cells, the so-called paracellular pathway. In addition, tight junctions limit the diffusion of lipids between the apical and basolateral plasma membrane domain. Claudin and Occludin, the two types of four-transmembrane domain proteins, are the major integral membrane components of the mammalian tight junction. So far, 20 distinct claudins have been identified. Mouse Claudin-2 was discovered in 1998 as a 230aa integral membrane protein localizing at tight junctions with an approximate molecular weight of 22.9kD. Claudin-1 and Claudin-2 are structurally related, and are in fact 38% identical at the amino acid sequence level. The expression of Claudin-2 is restricted to the liver and kidney, with small amounts found in the brain. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-3ug/ml. Mr: ~22-23kD. Reactivity has been confirmed with human, mouse, rat and dog liver and kidney homogenates, as well MDCK and Caco-2 cell lysates. A non-specific band at 27kD is observed in some lysates; in Caco-2 cells this band appears stronger than the correct band at 22kD. Immunhistochemistry (Paraffin): 1:10-1:100. Heat-induced epitope retrieval (HIER) with EDTA buffer, pH 8.0, is required prior to staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice