C5838-16-ML650
Claudin 2 (CLDN2) (MaxLight 650)
Size
£1,055.00
- SKU:
- C5838-16-ML650
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to a 26 amino acid sequence at the C-terminus of the mouse Claudin-2 protein.
- Clone:
- 3G95
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Tight Junctions (TJ) are specialized membrane domains that seal the intercellular spaces in epithelia and endothelia and thus contribute to the permeability barrier between lumenal and interstitial compartments. Tight junctions not only separate distinct physiological compartments, but they also confer selectivity to the transepithelial flux of molecules and ions through the intercellular spaces between the epithelial cells, the so-called paracellular pathway. In addition, tight junctions limit the diffusion of lipids between the apical and basolateral plasma membrane domain. Claudin and Occludin, the two types of four-transmembrane domain proteins, are the major integral membrane components of the mammalian tight junction. So far, 20 distinct claudins have been identified. Mouse Claudin-2 was discovered in 1998 as a 230aa integral membrane protein localizing at tight junctions with an approximate molecular weight of 22.9kD. Claudin-1 and Claudin-2 are structurally related, and are in fact 38% identical at the amino acid sequence level. The expression of Claudin-2 is restricted to the liver and kidney, with small amounts found in the brain. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: Reactivity has been confirmed with human, mouse, rat and dog liver and kidney homogenates, as well MDCK and Caco-2 cell lysates. A non-specific band at 27kD is observed in some lysates; in Caco-2 cells this band appears stronger than the correct band at 22 kDa. Immunhistochemistry (Paraffin): Heat-induced epitope retrieval (HIER) with EDTA buffer, pH 8.0, is required prior to staining. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice