CLC7 (Clcn7, Chloride Channel)

Size

£754.00

SKU:: C5837-52
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide 23aa sequence near the C-terminus of rat CLC-7 (KLH coupled). Species Sequence Homology: Mouse: 100%; human: 95%.
Format:: Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.09% sodium azide.
Extra Details:: Chloride is a critical component of all living cells. Voltage-gated chloride channels regulate cellular traffic of chloride ion. The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease and X-linked recessive nephrolithiasis. In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb). Additional forms of these proteins are obtained by alternative splicing. All CLC proteins (~700-1000aa) are predicted to contain 10 (possibly 12) transmembrane domains. CLC-1 and CLC-K1/K2 that are specific for kidney. Most other CLC are widely distributed in various tissues. Rat CLC-7 is 802aa membrane protein (mouse 803aa, human 805aa) (1). CLC-7 has a calculated MW of ~90kD. CLC-7 is ~45% identical with CLC-6. CLC-7 is expressed in brain, testes, muscle and kidney (1). Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml (ECL). CLC7 is ~80kD (2). ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Control Peptide: C5837-54: CLC7, Rat (Clcn7, Chloride Channel) Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice