C5835-07D

Clathrin Heavy Chain (CHC, CLHC, MRD56)

Size

£495.00

SKU:: C5835-07D
Purity:: Purified by Ion Exchange chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: BF-06
Format:: Supplied as a liquid in Tris, pH 8.0, 0.09% sodium azide.
Extra Details:: Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180kD) and three light chain (30kD) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis. Applications: Suitable for use in ELISA, Immunocytochemistry, Immunoprecipitation, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 4-10ug/ml. Intracellular staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.