C5818-01B-APC

Cks1 (Cdc kinase subunit 1) (APC)

Size

£1,010.00

SKU:: C5818-01B-APC
Purity:: Purified by Protein A affinity chromatography
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to the C-terminal region of human Cks1 protein.
Clone:: 6A41
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: Cks (Cdc kinase subunit) proteins are members of the cyclin-dependent kinase (cdk) family that act as adaptors to allow the cdks to interact more efficiently with their molecular targets. Cks1 is involved in the regulation of cell cycle mitosis in normal and cancer cells. Cks1 promotes mitosis by modulating the transcriptional activation of the APC/C protein-ubiquitin ligase activator Cdc20. Cks1 has also been established as an essential cofactor in the ubiquitylation of Cdk inhibitor p27 by Skp2 ubiquitin ligase. Mammalian Cks1 binds to Skp2 and promotes the association of Skp2 with phosphorylated p27 (Thr187), which further induces ubiquitylation of p27. High expression of Cks1 may be involved in the pathogenesis of human non-small cell lung carcinoma. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-3ug/ml Immunohistochemistry: 50ug/ml Note: Immunohistochemistry applications with formalin-fixed, paraffin-embedded tissue sections require heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0 Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell lysates and human tonsil Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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