C5073-05H
Ciliary Neurotrophic Factor, Recombinant, Human (CNTF)
Size
£552.00
- SKU:
- C5073-05H
- Molecular Weight:
- 22.8
- Purity:
- ≥98% by SDS PAGE and HPLC analysis. Endotoxin: ≤1EU/mg
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder from 10mM citric acid, pH 3.0., 75mM sodium chloride. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.. DO NOT VORTEX. Further dilutions can be made in a buffer with 0.1% HSA or BSA.
- Extra Details:
- CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types, including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity. Recombinant protein corresponding to human CNTF, synthesized as a 199 amino acid polypeptide (22.8kD) lacking a hydrophobic N-terminal signal for secretion, expressed in E. coli. Swiss/Uniprot: P26441 Molecular Weight: ~22.8kD Activity: Determined by its ability to stimulate proliferation of human TF-1 cells using an ED50 concentration range of 50-150ng/ml. Amino Acid Sequence: AFTEHSPLTP HRRDLCSRSI WLARKIRSDL TALTQSYVKH QGLNKNINLD SADGMPVAST DQWSQLTQAQ RLQQNLQAYR TFHVLLARLL QDQQVHFTPT QGDFHQAIHT LLLQVAAFAY QIQQLMILLQ YKIPRNQADG MPINVGDGGL FQKKLWGLKV LQQLSQWTVR SIHDLRFISS HQTGIPARGS HYIANNKKM Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice