C5073-05G

Ciliary Neurotrophic Factor (CNTF)

Size

£735.00

SKU:: C5073-05G
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human CNTF.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile ddH2O.
Extra Details:: CNTF mainly signals through binding to a receptor complex consisting of a CNTFRa subunit, two beta components, gp130 and LIFR, resulting ultimately in the activation of JAK/STAT signalling pathway, but CNTF has also been shown to bind to the receptor for interleukin-6 (IL-6R). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-2ug/ml Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.