C5072T
CIITA (Class II Trans Activator, C2TA, CIITA IV, MHC2TA, MHC Class II transactivator)
Size
£914.00
- SKU:
- C5072T
- Purity:
- Purified by ion exchange chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Peptide corresponding to a region near the C-terminal end (aa726-742) of the human CIITA gene conjugated to KLH. Sequence: CGEIKDNKELPQYLALTTR
- Format:
- Supplied as a sterile filtered liquid in PBS, pH 7.2, 0.015M sodium chloride, 0.01% sodium azide.
- Extra Details:
- CIITA and Regulatory Factor X5 (RFX5) are two transcriptional activators identified in human patients with bare lymphocyte syndrome. They bind to "X boxes" in class II MHC gene promoters. X boxes are conserved among class II MHC genes in humans and mice. The X box sequence is 5'-GTNRCC(N 0-3 )RGYAAC-3' where N is any nucleotide, R is a purine, and Y is a pyrimidine. Patients with bare lymphocyte syndrome have no class I or class II MHC molecules due to a complete lack of transcription of class I and class II MHC genes. Both class I and class II MHC genes are flanked by 5’ promoter sequences, which are bound by specific transcription factors. Consequences of Bare Lymphocyte Syndrome are:an absence of cellular and humoral T cell immune responses. Applications: Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 1:1000-1:2000 Western Blot: 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Note: Do not supplement buffers with Tween-20 detergent. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (25–50%) and freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice