C5072-01
CIDE-A, CT (Cell Death-inducing DFF-like Effector A)
Size
£655.00
- SKU:
- C5072-01
- Application:
- WB
- Purity:
- Immunoaffinity purified IgG
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Peptide corresponding to amino acids 200 to 217 of human CIDE-A (5).
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- Apoptosis is related to many diseases and induced by a family of cell death receptors. Cell death signals are transduced by DD-, DED-, or CARD-containing molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase DFF40/CAD, which is chaperoned and inhibited by DFF45/ICAD (1-4). DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified (5). CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD (5,6). Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is expressed in many tissues. This polyclonal antibody can be used for detection of CIDE-A by Western blot at 1:1000 to 1:2000 dilution. Human brain tissue lysate can be used as positive control and an approximately 23kD band can be detected. It has no crossreactivity to CIDE-B. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice