C5057-03
Choline Acetyltransferase (ChAT)
Size
£819.00
- SKU:
- C5057-03
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Chicken/Avian
- Immunogen:
- Synthetic peptide corresponding to Choline Acetyltransferase (KLH).
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
- Extra Details:
- Murine choline-O-acetyltransferase (EC 2.3.1.6) is a 71.721 kD protein (640aa) expressed in cholinergic neurons of both the PNS and CNS. In the CNS, ChAT is expressed in motor neurons and pre-ganglionic autonomic neurons of the spinal cord, a subset of neurons in the neostriatum, and in the basal forebrain. In the PNS, ChAT is expressed in a small subpopulation of sympathetic neurons and in all parasympathetic neurons. ChAT is the enzyme responsible for synthesis of acetylcholine from acetyl-coenzyme A and choline. Applications: Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:1000 -1:2000 Immunocytochemistry: 1:1000 -1:2000 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906 IgY, Chicken Pab Rb x Ch I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice