C5042-20A
Cholecystokinin A Receptor (CCKA Receptor, CCKAR, AW106902)
Size
£780.00
- SKU:
- C5042-20A
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from cell culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Gln428 from human Cholecystokinin A Receptor, expressed in NSO cells. Species sequence homology: Mouse, rat 89%.
- Clone:
- 7H167
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Cholecystokinin A Receptor (CCKAR) is one of two 7TM receptors for cholecystokinin peptide hormones. This GPCR is involved in gallbladder contraction, insulin secretion, and central nervous system function. CCK-A R has been implicated in pancreatic tumorigenesis as well as the pathogenesis of eating disorders and drug addiction. Human CCK-A R shares 89% aa sequence identity with mouse and rat CCKAR. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 25ug/ml, 10ul of the diluted solution labels 1-2.5x10e5 cells in a total reaction volume ≤200ul. Validated for use using human CCKAR transfectants. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice