C5042-20
Cholecystokinin A Receptor (CCKA Receptor, CCKAR, AW106902) (Not for Export EU)
Size
£1,138.00
- SKU:
- C5042-20
- Application:
- IHC-Fr
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide from the C-terminus of rat CCK-A receptor conjugated to BSA.
- Format:
- Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O. Dilute further with 0.1M PBS, 1% BSA, and 0.09% sodium azide.
- Extra Details:
- Cholecystokinin A Receptor (CCKAR) is one of two 7TM receptors for cholecystokinin peptide hormones. This GPCR is involved in gallbladder contraction, insulin secretion, and central nervous system function. CCK-A R has been implicated in pancreatic tumorigenesis as well as the pathogenesis of eating disorders and drug addiction. Human CCK-A R shares 89% aa sequence identity with mouse and rat CCKAR. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen sections): 1:1500-1:2500 using FITC conjugates as secondary antibodies; Incubation Time: Overnight at 2-8°C. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes staining. Opttimal dilutions to be determined by the researcher. Recommended Positive Control: Frozen rat pancreas sections. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice