C4500
Chemotaxis Inhibiting Protein of Staphylococcus aureus, CT (CHIPS)
Size
£991.00
- SKU:
- C4500
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Clone:
- JCC1
- Format:
- Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
- Extra Details:
- The bacterial pathogen Staphylococcus aureus is insensitive to antimicrobial host defense peptides such as defensins, protegrins, platelet microbicidal proteins and bacteriocins. Staphylococci have developed various resistance mechanisms including those specific for bacteriocins and several host defense peptides. A protein belonging to the resistance mechanism of Staphylococcus aureus is known as CHIPS (Chemotaxis Inhibiting Protein of Staphylococcus aureus). CHIPS is a protein produced by Staphylococcus aureus that inhibits chemotaxis of neutrophils by blocking the Formyl Peptide Receptor (FPR) and C5a Receptor on neutrophils. CHIPS and antibodies against CHIPS can be useful for various experimental infection models of Staphylococcus aureus. Furthermore these reagents can be of help in studies on the role of FPR and C5a in inflammatory processes. Applications: Suitable for use in Flow Cytometry, Western Blot, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10 Western Blot: 1:100 Immunohistochemistry (frozen and paraffin): 1:100 Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice