C4350-01L-PE

Chloramphenicol (Chloromycetin) (PE)

Size

£995.00

SKU:: C4350-01L-PE
Application:: FLISA
Purity:: ≥ 95% by SDS-PAGE. Purified from ascites by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Chloramphenicol-BSA conjugate
Clone:: 10i4
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with R-Phycoerythrin (PE).
Extra Details:: Chloramphenicol is a chlorine-containing antibiotic produced by Streptomyces venezuelae. It has a wide spectrum of activity against gram-positive and gram-negative cocci and bacilli (including anaerobes), Rickettsia, Mycoplasma and Chlamydia. It inhibits prokaryotic protein synthesis by attaching to the 50S ribosomal subunit. This inhibits peptidyltransferase, thereby preventing the formation of peptide bonds. It also inhibits protein synthesis in mitochondria which accounts for its toxic effects that cause aplastic anemia. Chloramphenicol has other adverse effects such as bone marrow depression and gray baby syndrome. It’s use is limited to serious infections such as those where resistance to other antibiotics occurs. It is still widely used in the treatment of typhoid fever, meningitis and eye infections. Applications: Suitable for use in competitive FLISA. Other applications have not been tested. Recommended Dilution: Optimal dilution to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells hybridized with spleen cells from BALB/c mice. Affinity Constant: Not determined Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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