C4275-35Q

Cholera Toxin, beta (Cholera Enterotoxin beta, Choleragenoid, CTX B, CTXB, CTX-B, TOXB, VC1456) (HRP)

Size

£541.00

SKU:: C4275-35Q
Purity:: Purified IgG fraction to a purity of ~95%.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Purified Choleragenoid.
Format:: Supplied as a liquid in PBS, 10mg/ml BSA, 0.002% thimerosal. Labeled with HRP. Estimated molar HRP:IgG ratio: 2-3.
Extra Details:: Cholera toxin is comprised of two subunits, A and B. Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration. The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1. Applications: Suitable for use in ELISA and Gel Immunoprecipitation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. End-Point Titer: >1:1000 by ELISA Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.