C4250-51F
Chlamydia LPS
Size
£628.00
- SKU:
- C4250-51F
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Chlamydia antigen
- Clone:
- 5F153 (ACI)
- Format:
- Supplied as a lyophilized powder from PBS, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
- Extra Details:
- LPS is a common feature of the outer envelope of gram negative bacteria, which acts as a potent endotoxin, triggering an innate immune response. Studies have shown however, that whilst the LPS of Chlamydia trachomatis does evoke an immune response, it displays only weak endotoxic activity when compared to that of other bacteria such as Salmonella Minnesota or Neisseria gonorrhoeae. Applications: Suitable for use in Immunofluorescence Microscopy, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilutions: Immunofluorescence Microscopy: 1:10 with PBS, pH 7.4. Immunohistochemistry: Paraformaldehyde-fixed tissue sections. Detects chlamydia species in samples after fixation with methanol/acetone (1:1). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder and reconstituted products must be stored at 4°C. Reconstitute with sterile ddH2O. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice