C4154-20B

Chitinase, Streptomyces griseus (Chitodextrinase; Poly(1,4-beta-[2-acetamido-2-deoxy-D-glucoside]) glycanohydrolase)

Size

£646.00

SKU:: C4154-20B
Purity:: Chitinase Activity: ≥200 units/g solid
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder (almost salt-free). Reconstitute with 50mM potassium phosphate, pH 6.0 yields a clear to hazy mixture.
Extra Details:: Chitinase is an extracellular enzyme complex that degrades chitin. It has been detected in many microorganisms and also in the digestive tracts of many animals that feed on chitin-containing organisms. The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine is performed by two consecutive enzyme reactions. The first one, chitodextrinase-chitinase is a poly(1,4-β-[2-acetamido-2-deoxy-D-glucoside])-glycanohydrolase (EC. 3.2.1.14), which removes chitobiose units from chitin. The second one is N-acetyl- glucosaminidase-chitobiase, which cleaves the disaccharide to its monomer subunits, N-acetyl-D-glucosamine (NAGA). The optimal reaction temperature is 37°C. Chitinase Activity: ≥200 units/g solid Protein Content: As Reported Unit Definition: One unit will liberate 1mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25°C in a 2 hour assay. Storage & Stability: Lyophilized powder may be stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.