C3890-01

Chemerin (TIG2)

Size

£825.00

SKU:: C3890-01
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Glu21-Ser157 of human Chemerin expressed in E. coli
Clone:: 7H141
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Chemerin, also known as TIG2, is a distant member of the cystatin superfamily. The chemerin precursor is cleaved by multiple serine proteases at various sites near the C-terminus. Mature active chemerin is a 15-16kD heparin binding protein that induces immature dendritic cell and macrophage chemotaxis through interactions with ChemR23. Mature human chemerin shares 65% and 70% aa sequence identity with mouse and rat chemerin, respectively. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml using human kidney tissue Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.