Chapsyn 110 (PSD93, Discs Large Homolog 2, DLG2, Channel-associated Protein of Synapses), (MaxLight 650)

Size

£914.00

SKU:: C3550B-ML650
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide derived from the mid region of PSD-93 protein.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Extra Details:: MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. PSD-93 (Post Synaptic Density 93) belongs to a class of proteins referred to as MAGUKs (membrane-associated guanylate kinases). MAGUKs are widely expressed in the brain and are critical elements of the cytoskeleton and of certain synapses. Cannonical MAGUK proteins contain one or three PDZ (PSD-95/discs large/zona occludens-1) domains, an SH3 domain, and a guanylate kinase homology domain. These proteins appear to regulate synaptic function by acting as molecular scaffolds to organize and cluster signaling machinery at synapses, in part by interaction of their PDZ domains with carboxy-terminal T/SXV motifs present on synaptic proteins including NMDA receptors and Shaker type K+ channels. PSD-93 and several other MAGUK and PDZ containing proteins (eg. PSD-95, SAP-97, SAP-102, MALS/Veli proteins,) are enriched in the post synaptic density. PSD-93 is expressed in discrete neuronal populations as well as in specific nonneuronal cells and exhibits complex molecular diversity attributable to tissue-specific alternative splicing. PSD-93, like PSD-95, binds to nNOS and to the NMDA receptor 2B. PSD-93, however, is unique among PSD-95/SAP-90 family members in its expression in cerebellar Purkinje neuron cell bodies and dendrites. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilution: Immunofluorescence: 1-3ug/ml Immunoprecipitation: 5-10ug/IP reaction Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Positive control: rat brain. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice