Ceruloplasmin (CP, EC 1.16.3.1, Ferroxidase)

Size

£776.00

SKU:: C2900-07A
Purity:: Purified affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Ceruloplasmin isolated and purified from human serum
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, dextran. Reconstitute with 500ul sterile ddH2O. Dilute further in PBS, pH 7.2.
Extra Details:: Ceruloplasmin (Cp) is a plasma metalloprotein synthesized and secreted by the liver. Cp is a blue alpha-2-glycoprotein that binds 90 to 95% of plasma copper and has 6 or 7 cupric ions per molecule. It is involved in peroxidation of Fe(II) transferrin to form Fe(III) transferrin. Human Cp is a single polypeptide chain (1,065aa, 135kD; chromosome 8q21.13-q23.1) structurally similar to factors V and VIII. Cp also has abundant expression in specific populations of glial cells within the brain. Hereditary Cp deficiency have profound iron accumulation in most tissues. Cp -/- mice showed no abnormalities in cellular iron uptake but a striking impairment in the movement of iron out of reticuloendothelial cells and hepatocytes. Cp may play an essential physiologic role in determining the rate of iron efflux from cells with mobilizable iron stores. Applications: Suitable for use in ELISA, Western Blot, Immunoelectrophoresis, Single Radial Immunodiffusion and Enzyme Inhibition. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice