CENP-Q (Centromere Protein Q)

Size

£914.00

SKU:: C2615-25
Molecular Weight:: 2631
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Full-length human CENP-Q recombinant protein.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Extra Details:: Cenp-Q (also known as centromere protein Q or CENPQ) is a nuclear/centromeric protein that is one of the critical components that constitutes the CENP-O complex at the kinetochores and appears to stabilize PBIP1/CENP-U(50)/MLF1IP in the complex. This complex is important for proper recruitment of polo-like kinase 1 (Plk1) to the mitotic kinetochores. A failure in this process results in improper microtubule attachment to the kinetochores and chromosome missegregation that ultimately lead to aneuploidy. Applications: Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100-1:500 ELISA: 1:20,000–1:30,000 Western Blot: 1:2,000-1:2,500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.