C2615-10

CENP-A (Centromere Protein A)

Size

£819.00

SKU:: C2615-10
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to a portion of human CENP-A.
Clone:: 4E43
Format:: Supplied as a liquid in PBS, pH 7.2, 50% glycerol.
Extra Details:: Centromere protein A (CENP-A) is a 17-19kD centromere-specific histone variant that is 62% identical to the carboxy-terminal domain of histone H3. In the presence of DNA CENP-A forms an octameric complex with histones H4, H2A, and H2B. CENP-A defines active centromere regions by forming centromere-specific nucleosomes on which kinetochores are assembled. CENP-A is specifically located to the inner plate of the kinetochore and it is essential for kinetochore targeting of CENP-C and other kinetochore components. CENP-A is required for nucleosomal packaging of centromeric DNA at interphase. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Immunohistochemistry: 5ug/ml Immunocytochemistry: 10ug/ml Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.