C2589-90B
CEBP alpha, phosphorylated (Ser21) (CCAAT Enhancer Binding Protein, alpha)
Size
£639.00
- SKU:
- C2589-90B
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phospho-peptide corresponding to residues surrounding serine 21 of human C/EBPalpha
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol
- Extra Details:
- CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal function and inflammatory response (1). Six members of the family have been characterized (C/EBPalpha,-beta,-delta,-gamma,-epsilon and-zeta) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPalpha (p42 and p30), both are strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-1 stimulate the dephosphorylation of C/EBPalpha, which may play a key role in insulin induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPalpha at Thr222, Thr226 and Ser230 by GSK3 seems to be required for adipogenesis (4). Applications: Suitable for use in Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
- Shipping Conditions:
- Blue Ice