C2589-90
CEBP beta, phosphorylated (CCAAT Enhancer Bnding Protein beta)
Size
£581.00
- SKU:
- C2589-90
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues surrounding Ser105 of rat C/EBP beta.
- Format:
- Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
- Extra Details:
- CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal functions and inflammatory response (1). Six members of the family have been characterized (C/EBP alpha, C/EBP beta, C/EBP delta, C/EBP epsilon, C/EBP gamma and C/EBP zeta) and are distributed in a variety of tissues (1). Translation from alternative start codons results in three isoforms of C/EBP beta. Two of them are transcriptional activators, also known as p35 and p32 LAPs (liver activating proteins). The smaller isoform, p20 LIP (liver inhibitory protein), however, is a transcriptional inhibitor (2). Phosphorylation of C/EBP beta at distinct sites stimulates its transcriptional activity (3–5). Phosphorylation at serine 105 of rat C/EBP beta, a unique site only present in the rat sequence, seems essential for rat C/EBP beta activation (6). Applications: Suitable for use in ELISA and Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice