C2589-68

CEACAM 3 (Carcinoembryonic Antigen Related Cell Adhesion Molecule 3, CD66d, CGM1)

Size

£812.00

SKU:: C2589-68
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Lys35-Gly155 of human CEACAM 3 expressed in NS0 cells.
Clone:: 7H213
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Carcinoembryonic Antigen-related Cell Adhesion Molecule 3, (CEACAM3) also known as CD66d and CGM1, is a 35kD transmembrane glycoprotein in the CEACAM family of the immunoglobulin superfamily. The extracellular region consists of one Ig-like V-type domain, and the cytoplasmic tail contains one ITAM. CEACAM3 is upregulated on activated neutrophils and mediates their adhesion to vascular endothelial cells. CEACAM3 binds the Opa opacity proteins on some Gram negative bacteria, leading to bacterial uptake and neutrophil death. Alternate splicing generates an isoform with a substituted cytoplasmic tail and a potentially secreted isoform that contains a highly charged and Ser/Thr-rich sequence in place of the transmembrane and cytoplasmic regions. Orthologs of human CEACAM3 have not been identified in other species. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice