C2585-28
CDw49f (VLA6, ITGA6, ITGA6B) (FITC)
Size
£767.00
- SKU:
- C2585-28
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Cell line SW/222 (Human colorectal carcinoma).
- Clone:
- 2Q959 (4F10)
- Format:
- Supplied as a liquid in PBS, 0.1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
- Extra Details:
- CDw49f is expressed on epithelial and endothelial basement membrane. The VLA alpha-6 chain, which consists of a single disulfide linked 120kD subunit and a small doublet subunit of 25/30kD, is non-covalently associated with the VLA beta-chain (CD29) in VLA-6 or alpha 6/beta 1 complex or with integrin beta 4 chain (alpha 6/beta 4). The antigen is also expressed on platelets, monocytes, thymocytes and T lymphocytes. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice