C2585-25B
CD49f (ITGA6, Integrin, alpha 6, CD49wf antigen, Integrin alpha-6 precursor, VLA-6)
Size
£863.00
- SKU:
- C2585-25B
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant human Integrin alpha 6 aa93-148; aa55-613 (E. coli).
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Integrin alpha 6 (also VLA-6, CD49F and ITA6) is a 130-150kD member of the Integrin alpha chain family of molecules. Mature Integrin alpha 6 is a 1107aa type I transmembrane glycoprotein that contains a 1027aa extracellular region and a 93 aa cytoplasmic tail. The ECD is cleaved to generate a disulfide-linked dimer that shows a 125kD heavy chain (aa24-938), and a 30-31kD membrane-bound light chain (aa942-1130). Integrin alpha 6 forms a nondisulfide-linked heterodimeric complex with both Integrin beta1 and beta4. Each complex serves as a receptor for laminin. Splice variants exist that show either a deletion of aa215-258 or a deletion of aa260-298, possibly accompanied by a 29aa substitution for aa1084-1130. Over aa93-148 and 518-574, human Integrin alpha6 shares 89% aa identity with mouse Integrin alpha6. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice