C2584-01B
CD17 (CDw17)
Size
£595.00
- SKU:
- C2584-01B
- Application:
- Flow Cytometry
- Purity:
- Purified by ammonium sulfate precipitation followed by gel filtration.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Pre-B cell line NALM-6
- Clone:
- MEM-68
- Format:
- Supplied as a liquid in TBS, pH 7.4, 15mM sodium azide.
- Extra Details:
- CDw17 / CD17 is an intermediate glycosphingolipid from the metabolism of higher gangliosides that localizes to sphingolipid-sterol rafts. CD17 is detectable in monocytes, granulocytes, basophils, platelets, a subset of peripheral B cells (CD19 ) and tonsil dendritic cells. It is rapidly down regulated on activated granulocytes and is upregulated on IL-2 activated T lymphocytes. CD17 binds to bacteria and may function in phagocytosis. VEGF-treated endothelial cells can produce CD17, which can then mediate signaling toward PECAM-1 expression and angiogenesis. Tumor necrosis factor alpha (TNF-alpha)-induced astrogliosis (astrocyte proliferation and glial fibrillary acidic protein upregulation) in response to neuro-inflammation (i.e. spinal cord injury) causes an increase in intracellular levels of CD17. Aberrant levels of glycosphingolipids are a feature of cancer cells and may influence integrin clustering and internalization. C2584-01B is useful for identification of lactosylceramide expressing cell populations. After an neuraminidase treatment all PBC become positive. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice