C2575-01-ML650
cdk5 (Cyclin-dependent p31) (MaxLight 650)
Size
£1,023.00
- SKU:
- C2575-01-ML650
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human cdk5.
- Clone:
- 4i34 (DC17)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation and FLISA. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml detects cdk5 in a RIPA lysate from non-stimulated human A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C2575-01 (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots of antibody detected cdk5 in RIPA lysates from mouse Wehi cells and rat PC12 cells. Immunoprecipitation: 4ug/500ug of A431 cell lysate. Immunocytochemistry: 5ug/ml stains cdk5 in human A431 cells fixed with 4% paraformaldehyde. Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: A0001-50 A431 Cells, Non-Stimulated. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice