C2574-55

cdk4 (Cyclin-dependent Kinase 4)

Size

£761.00

SKU:: C2574-55
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant human cdk4 protein.
Clone:: 2Q306 (DCS-35)
Format:: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Extra Details:: The prototype of the cdk family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression. Applications: Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Kinase Assay. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml for 2 hrs at RT. Immunoprecipitation: Excellent for coprecipitation of D-type cyclins bound to cdk4. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.