C2574-05

cdk4, p34 (CDK4, Cyclin-dependent Kinase 4, p34cdk4)

Size

£927.00

SKU:: C2574-05
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant cdk4 protein. Cellular Localization: Nuclear and cyotplasmic. Epitope: aa1-20.
Clone:: 0.N.199 (DCS-35)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Extra Details:: A family of proteins designated as cdks are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Kinase assays. Other applications have not been tested. Recommended Dilution: Immunoprecipitation Native only; coprecipitates cyclin D’s: 2ug/mg protein lysate. Use protein G Western Blot: 1-2ug/ml for 2 hours at RT. Optiml dilutions to be determined by the researcher. Positive Control: MAD109, NIH3T3, LS174T, and MCF-7 cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.