C2572-31

cdk2 (Cyclin-dependent Kinase 2)

Size

£800.00

SKU:: C2572-31
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated, synthetic peptide corresponding to aa287-298 (CQDVTKPVPHLRL) of human cdk2, with a cysteine added for conjugation purposes.
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Extra Details:: Applications: Suitable for use in Western Blot, Immunoprecipitation and IP-kinase assay. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml detected cdk2 in nuclear extracts from HeLa cells. Immunoprecipitation: 2-4ug immunoprecipitated cdk2 from 500ug of HeLa nuclear extract. Immunoprecipitation Kinase Assay: 4ug \immunoprecipitated active cdk2 from HeLa nuclear extract as shown by the ability to phosphorylate Histone H1. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.