C2572-29

cdk2, phosphorylated (Thr160) (Cyclin-dependent Kinase 2)

Size

£639.00

SKU:: C2572-29
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phosphopeptide corresponding to residues surrounding Thr160 of human cdk2.
Format:: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Extra Details:: Cyclin-dependent kinase 2, p33cdk2, is an important component of the cell cycle machinery. Like p34cdc2, its kinase activity is regulated by association with a cyclin subunit, by its phosphorylation state and by association with a cdk inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15. Inhibition of cdk2-cyclin complexes can also be attributed to association with p27Kip1 and p21Waf1/Cip1. Activation of cdk2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160, which is mediated by CAK, a complex of cdk7 and cyclin H. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA (Peptide): 1:2000 Flow Cytometry: 1:25 Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:150 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice