C2572-07
cdk2 (Cyclin-dependent Kinase 2)
Size
£825.00
- SKU:
- C2572-07
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human cdk2.
- Clone:
- 4i33 (AN4.3)
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
- Extra Details:
- p33 cdk2 associates with cyclin A in human cells. Kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1 and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle. It is important (as does cyclin A-cdk2) for the progression from G1- to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition. Both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S- and G2-phase. Applications: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 4ug Immunoprecipitates cdk2 from 500ug of mouse 3T3 nuclear extract. Western Blot: 0.5-2ug/ml. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with C2572-07. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Positive Antigen Control (Included): H1838: HeLa Nuclear Extract Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receiot. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice