C2549-50P
cdc2, p34
Size
£787.00
- SKU:
- C2549-50P
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein consisiting of the carboxy-terminal two-thirds of the Xenopus p34cdc2.
- Clone:
- 3G92
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Inhibition and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1ug/ml Western Blot: 0.5-1ug/ml Immunoprecipitation: 5ug. Capable of immunoprecipitating cyclin protein associated with p34cdc2. Inhibition: Inhibits the activation of p34cdc2 kinase by cyclin proteins. Immunohistochemistry (Frozen): 5-10ug/ml. Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: A431 and HeLa 3T3 cell lysates; Xenopus egg extracts. Immunohistochemistry: Tested on a variety of human tissues including tonsil (germinal center), spermatogonia and skin (basal layer). Epitope Location: The epitope core residues are thought to reside within amino acids 220-227 of the mouse p34cdc2 protein corresponding to the sequence LGTPNNEV. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice