C2549-50P-ML490

cdc2, p34 (MaxLight 490)

Size

£971.00

SKU:: C2549-50P-ML490
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein consisiting of the carboxy-terminal two-thirds of the Xenopus p34cdc2.
Clone:: 3G92
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
Extra Details:: MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Applications: Suitable for use in FLISA, Western Blot, Immunoprecipitation, Inhibition and Immunohistochemistry. Other applications not tested. Recommended Dilution: FLISA: 0.1-1ug/ml Western Blot: 0.5-1ug/ml Immunoprecipitation: 5ug. Capable of immunoprecipitating cyclin protein associated with p34cdc2. Inhibition: Inhibits the activation of p34cdc2 kinase by cyclin proteins. Immunohistochemistry (Frozen): 5-10ug/ml. Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: A431 and HeLa 3T3 cell lysates; Xenopus egg extracts. Immunohistochemistry: Tested on a variety of human tissues including tonsil (germinal center), spermatogonia and skin (basal layer). Epitope Location: The epitope core residues are thought to reside within amino acids 220-227 of the mouse p34cdc2 protein corresponding to the sequence LGTPNNEV. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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