C2549-50D

cdk1, p34cdc2 (CDC2, Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, Cell division control protein 2 homolog, Cyclin-dependent kinase 1, p34 protein kinase)

Size

£761.00

SKU:: C2549-50D
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Xenopus p34cdc2. Cellular Localization: Nuclear with some cytoplasmic staining
Clone:: 2Q305
Format:: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Extra Details:: Cdk1/p34cdc2 protein kinase is a catalytic subunit of Maturation Promoting Factor. p34cdc2, a serine/threonine kinase, is activated by cyclins, presumably by dephosphorylation of tyrosine residues. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot and Kinase Assay. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation (Native, denatured): 2ug/mg protein lysate using Protein A. Immunohistochemistry (Formalin/paraffin, frozen): 2-4ug/ml for 30 minutes at RT. Paraffin sections require antigen retrieval. Western Blot: 2ug/ml for 2 hours at RT. Optimal dilution determined by the researcher. Positive Controls: HeLa or NIH3T3 cells; germinal center in a tonsil or reactive lymph node. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.