C2549-29A
CD301 (CD301 Antigen, C-type Lectin Domain Family 10 Member A, CLEC10A, CLECSF13, C-type Lectin Superfamily Member 14, CLECSF14, HML2, Macrophage Lectin 2)
Size
£812.00
- SKU:
- C2549-29A
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Balb/c macrophage precursor cell hybrids.
- Clone:
- 10B1349 (ER-MP23)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- CD301 is a 38kD cell surface protein that is otherwise known as macrophage galactose N-acetylgalactosamine lectin (MGL) or dendritic cell asialoglycoprotein (DC-ASGPR). In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Expression of CD301 is induced by alternative (i.e. IL-4/IL-13 mediated) activation of macrophages and dendritic cells, but not all CD301 positive cells are necessarily IL-4/IL-13 stimulated. Applications: Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): Heat mediated antigen retrieval is required prior to staining paraffin-embedded sections using citrae buffer, pH 6.0. Optimal dilutions to be determined by the researcher. Hybridoma: Rat Y3-Ag1.2.3 myeloma cells fused with cells from immunized rats. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice